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Related Experiment Videos

Interferon induction: regulation by both virus and cell

P I Marcus1, M J Sekellick

  • 1Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3004.

[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|November 1, 1994
PubMed
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Viral and host cell factors significantly regulate interferon (IFN) production. Vesicular stomatitis virus (VSV) and chick embryo cells demonstrate how viruses and cells control IFN induction and yield.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Interferon (IFN) induction and production are critical immune responses regulated by both viral and host factors.
  • Viruses can modulate IFN responses by altering IFN inducer delivery (dsRNA) or suppressing IFN gene expression.
  • Host cells also regulate IFN production during development and physiological changes like aging in vitro.

Purpose of the Study:

  • To systematically define the regulatory roles of viruses and host cells in IFN induction and production.
  • To investigate the range of IFN yields influenced by genetic and physiological variations in both virus and cell.
  • To utilize vesicular stomatitis virus (VSV) and chick embryo cells as model systems.

Main Methods:

  • Comparative analysis of IFN induction and production across different viral strains and host cell conditions.

Related Experiment Videos

  • Assessment of IFN yields in developing chicken embryos and aged in vitro cell cultures.
  • Examination of viral suppression of IFN induction and the genetic basis of IFN inducibility.

    • Main Results:

    • Viral regulation of IFN induction can vary up to 10,000-fold, with some viruses actively suppressing IFN production.
    • Host cell regulation, observed in developing embryos and aged cell lines, shows a 1,000-fold to 10-fold range in IFN inducibility.
    • IFN regulatory factors are themselves subject to regulation during cellular development and aging.

    Conclusions:

    • Both viral and host cell components exert significant, quantifiable control over IFN induction and production.
    • Understanding these regulatory mechanisms is key to comprehending innate immune responses.
    • VSV and chick embryo cell models provide valuable insights into the complex interplay governing IFN responses.