Related Experiment Video
Updated: Aug 14, 2026
13:27
Generation of Human CD40-activated B cells
Published on: October 16, 2009
pp60c-src expression is induced by activation of normal human T lymphocytes
1Research Section, Canadian Red Cross Society, Toronto, Ontario.
Journal of Immunology (Baltimore, Md. : 1950)
|April 15, 1995
Summary
The cellular homologue pp60c-src is present in human T cell lines and thymocytes. Its expression is induced in peripheral blood T lymphocytes upon activation, suggesting a role in T cell signaling.
Area of Science:
- Immunology
- Molecular Biology
- Cellular Signaling
Background:
- pp60c-src is the normal cellular homologue of the Rous sarcoma virus transforming protein.
- Its presence and role in human T cells, particularly during activation, require further investigation.
Purpose of the Study:
- To determine the presence and expression levels of pp60c-src in various human T cell populations.
- To investigate the regulation of pp60c-src expression following T cell activation.
Main Methods:
- In vitro immune-complex kinase assay
- Western blotting using anti-pp60c-src monoclonal antibodies (mAbs) 327 and GD11
- RNase protection assays
- Flow cytometry analysis of T cell subsets (CD4+ and CD8+)
Main Results:
- pp60c-src protein and mRNA are present in T cell lines (e.g., Jurkat) and freshly isolated thymocytes.
- pp60c-src is largely absent in resting peripheral blood T lymphocytes but is induced upon activation via PHA or TCR stimulation.
- Activated CD4+ and CD8+ T cells show peak pp60c-src expression 12-24 hours post-activation.
- Significant pp60c-src kinase activity is detected in thymocytes, activated T cells, and T cell lines.
Conclusions:
- pp60c-src is constitutively expressed in some T cell lines and thymocytes, and inducibly expressed in peripheral T cells upon activation.
- The induction of pp60c-src following T cell activation suggests a potential role in T cell receptor (TCR) signaling pathways.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:27Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...