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All you wanted to know about SELEX

S J Klug1, M Famulok

  • 1Institut für Molekulare Biochemie, Genzentrum der Ludwig-Maximillian-Universität Müchen, Germany.

Molecular Biology Reports
|January 1, 1994
PubMed
Summary

In vitro selection, also known as SELEX, is a powerful method for screening diverse nucleic acid libraries to identify molecules with specific functions. This technique offers broad applications and potential solutions for overcoming limitations in molecular discovery.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biochemistry

Background:

  • In vitro selection, or SELEX, is a key technique for identifying functional nucleic acid molecules.
  • It enables the screening of vast libraries of DNA or RNA molecules simultaneously.

Purpose of the Study:

  • To provide a comprehensive overview of the in vitro selection (SELEX) method.
  • To illustrate the diverse applications and potential of SELEX through conclusive examples.
  • To discuss limitations and strategies for overcoming them.

Main Methods:

  • In vitro selection (SELEX) involves iterative cycles of selection, amplification, and diversification.
  • The method can be applied to both RNA and DNA (single-stranded or double-stranded) molecules.
  • Variations of the SELEX technique are explored.

Main Results:

  • SELEX allows for the identification of molecules with specific binding or catalytic properties.
  • Numerous successful applications across various scientific fields are demonstrated.
  • The power and versatility of SELEX in molecular discovery are highlighted.

Conclusions:

  • In vitro selection (SELEX) is a robust and versatile methodology for discovering functional nucleic acids.
  • Understanding its variations and limitations is crucial for successful application.
  • SELEX holds significant potential for advancing various areas of biological and medical research.

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