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Preprotachykinin-A and substance P receptor (NK1) gene expression in rat astrocytes in vitro
H P Too1, D R Marriott, G P Wilkin
1Department of Biochemistry, National University of Singapore, Kent Ridge Crescent.
Neuroscience Letters
|December 5, 1994
Abstract:
The presence of mRNA transcripts coding for preprotachykinin-A and the substance P receptor in cultured astrocytes is demonstrated by a combination of reverse transcription/polymerase chain reaction (PCR) and Southern blotting. These findings showed that astrocytes in culture are capable of synthesing both the precursor of substance P (preprotachykinin-A) and the cognate receptor, substance P receptor (NK1). The simultaneous presence of both the ligand (substance P) and the receptor (NK1) may indicate an autocrine nature of astrocyte communication.