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A neutrophil-derived NO-synthase (NOS) inhibitor
A Dembinska-Kiec1, M Burchert, J Hartwich
1Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland.
Agents and Actions. Supplements
|January 1, 1995
Abstract:
In the present work we have demonstrated that the low NOS activity of circulating blood neutrophils is related to an endogenous inhibitory factor. This factor inhibits constitutive NOS (cNOS) of cerebellum and inducible NOS (iNOS) of macrophages in a concentration-dependent manner. Boiling only partially diminished its activity. The inhibition of cNOS was specific, since to some degree NADPH (0.5-4 mM) and more effectively L-arginine (0.1-1 mM), but not D-arginine, reversed the inhibition.