[Preparation and application of ELISA kit for detection of G-CSF]

By adding monoclonal antibodies from BALB/c mice to the granulocyte colony-stimulating factor (G-CSF), we established a sandwich ELISA for detecting G-CSF levels in human serum samples. G-CSF was measured in 157 normal serum samples and in 153 serum samples from patients with no clinical manifestation of bacterial infection and in 269 serum samples from patients with clinical diagnosis of bacterial infection and in 31 serum samples from patients with positive bacterial infection according to cell culture. It was found that the positive rates of G-CSF were zero in normal serum samples, only 6.5% in patients with no clinical manifestation of bacterial infection, 89.2% in patients with clinical diagnosis of bacterial infection and 100% in patients with bacterial culture positive for infection. Our results reveal that the sandwich ELISA for detection of G-CSF levels in human serum samples may be useful for diagnosing patients with bacterial infection and for clinically guiding the rational use of antibiotics.