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Induction of calcium-independent nitric oxide synthase activity in cultured cerebellar granule neurons
1Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Neuroscience Letters
|January 23, 1995
Abstract:
Cultured cerebellar granule neurons were assayed for nitric oxide synthase (NOS) activity by measuring the conversion of L-arginine to L-citrulline. Granule neurons expressed constitutive NOS activity which was calcium-dependent. Treatment of neuron cultures for 24 h with the combined stimulation of IFN-gamma plus IL-1 beta, TNF-alpha and LPS induced NOS activity by 87-fold which was calcium-independent. We conclude that cultured cerebellar granule neurons can express both the constitutive and inducible forms of NOS activity.