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Three isoforms of platelet-derived growth factors all have the capability to induce angiogenesis in vivo
T Oikawa1, C Onozawa, M Sakaguchi
1Department of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan.
Insights
Platelet-derived growth factors (PDGFs) BB, AB, and AA isoforms show dose-dependent angiogenic activity. Their indirect mechanism suggests PDGFs are not direct stimulators of vascular endothelial cell proliferation.
Area of Science:
- Biochemistry
- Molecular Biology
- Developmental Biology
Background:
- Platelet-derived growth factors (PDGFs) are crucial signaling molecules involved in cell growth and differentiation.
- Understanding the specific roles of different PDGF isoforms (AA, AB, BB) in vascular development is essential.
Purpose of the Study:
- To investigate the angiogenic activity of the three PDGF isoforms (AA, AB, BB).
- To determine if PDGF isoforms directly stimulate vascular endothelial cell proliferation.
Main Methods:
- In vivo assay using the chorioallantoic membrane of chick embryos to assess angiogenic activity.
- Dose-dependent evaluation of PDGF-AA, PDGF-AB, and PDGF-BB.
- Assessment of vascular endothelial cell proliferation.
Main Results:
- All three PDGF isoforms (AA, AB, BB) demonstrated dose-dependent angiogenic activity.
- The order of potency for angiogenic activity was determined as PDGF-BB > PDGF-AB = PDGF-AA.
- PDGF isoforms did not directly stimulate the proliferation of vascular endothelial cells.
Conclusions:
- The three PDGF isoforms exhibit angiogenic effects through an indirect mechanism.
- PDGFs likely act on other cell types or signaling pathways to promote angiogenesis, rather than directly on endothelial cells.
Abstract:
Three isoforms of platelet-derived growth factors (PDGFs) composing of AA, AB, and BB chains all exhibited angiogenic activity in a dose-dependent manner in an in vivo assay system involving the chorioallantoic membrane of chick embryo. The order of potency as BB > AB = AA. They, however, failed to stimulate proliferation of vascular endothelial cells, suggesting that their effects are indirect. These data suggest that possibility that three PDGF isoforms are indirect angiogenic factors.