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Related Experiment Videos

The E-cadherin complex contains the src substrate p120

D F Aghib1, P D McCrea

  • 1Department of Growth and Development, University of California, San Francisco 94143, USA.

Experimental Cell Research
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

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This study reveals new protein interactions within the E-cadherin complex, identifying novel components and their potential roles in cell adhesion and signaling pathways.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • E-cadherin is a crucial transmembrane protein mediating calcium-dependent cell-cell adhesion.
  • The E-cadherin complex includes cytoplasmic proteins like alpha-catenin, beta-catenin, plakoglobin, and actin.
  • Understanding the E-cadherin complex composition and regulation is vital for cell adhesion studies.

Purpose of the Study:

  • To investigate the composition and tyrosine phosphorylation of the E-cadherin complex.
  • To identify novel protein interactions and components within the E-cadherin complex.
  • To explore the role of src kinase in modulating E-cadherin complex composition and phosphorylation.

Main Methods:

  • Utilized normal and temperature-sensitive viral src allele (ts-src) transfected MDCK cells.

Related Experiment Videos

  • Employed immunoprecipitation and Western blot analysis to examine protein complexes.
  • Investigated protein interactions and tyrosine phosphorylation status within the E-cadherin complex.

    • Main Results:

    • Identified two heterodimeric complexes: alpha-catenin with beta-catenin, and alpha-catenin with plakoglobin.
    • Discovered three new p120-related protein components associated with the E-cadherin complex.
    • Observed increased p120-related proteins in ts-src MDCK cells and significant tyrosine phosphorylation of two species.

    Conclusions:

    • E-cadherin likely associates with the actin cytoskeleton via beta-catenin or plakoglobin.
    • The newly identified p120-related proteins may modulate E-cadherin function.
    • The alpha-catenin-beta-catenin and alpha-catenin-plakoglobin dimers might play roles in regulating catenin availability for complexes like APC.