Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Talin does not associate exclusively with alpha 2b beta 3 integrin in activated human platelets

G Escolar1, M Diaz-Ricart, J G White

  • 1Hospital Clínico y Provincial, Barcelona, Spain.

The Journal of Laboratory and Clinical Medicine
|May 1, 1995
PubMed
Summary

Talin protein redistributes to the platelet submembrane region upon activation, but does not exclusively colocalize with glycoprotein IIb-IIIa (GPIIb-IIIa). This suggests talin may stabilize connections with other platelet glycoproteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A freeze-dried platelet-derived hemostatic agent improves hemostasis in patients with severe thrombocytopenia before and after standard platelet transfusion: Studies ex vivo in the total thrombus-formation analysis system 01.

Transfusion·2026
Same author

Diagnostic challenges in von Willebrand disease. Report of two cases with emphasis on multimeric and molecular analysis.

Platelets·2020
Same author

Towards the understanding of the UV light, riboflavin and additive solution contributions to the in vitro lesions observed in Mirasol®-treated platelets.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2019
Same author

Emicizumab for routine prophylaxis to prevent bleeding episodes in patients with hemophilia A.

Drugs of today (Barcelona, Spain : 1998)·2018
Same author

Incomplete reversibility of platelet inhibition following prolonged exposure to ticagrelor: comment.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Betrixaban: a direct oral inhibitor of activated factor X for the prophylaxis of venous thromboembolism in patients hospitalized for acute medical illness.

Drugs of today (Barcelona, Spain : 1998)·2017

Area of Science:

  • Platelet biology
  • Cellular adhesion
  • Protein biochemistry

Background:

  • Talin is a protein hypothesized to link cytoplasmic actin to the submembrane glycoprotein IIb-IIIa (GPIIb-IIIa) complex in activated human platelets.
  • Understanding talin's role is crucial for comprehending platelet function and hemostasis.

Purpose of the Study:

  • To investigate the association of talin with the platelet cytoskeleton during thrombin-induced activation.
  • To determine the precise localization of talin relative to activated glycoprotein receptors.

Main Methods:

  • Morphologic and electrophoretic techniques were employed.
  • Immunogold cytochemistry was used to localize talin in ultrathin cryosections of platelets.
  • Studies included normal platelets and those with defects in GPIIb-IIIa (Glanzmann's thrombasthenia) and GPIb (Bernard-Soulier syndrome).

Related Experiment Videos

Main Results:

  • Activated platelets showed fibrinogen-coated gold particles (Fgn/Au) bound to the surface, with talin localizing to the submembrane region.
  • Immunoblots indicated GPIIIa, but not talin, associated with activated platelet cytoskeletons.
  • Talin redistribution to the submembrane region occurred independently of GPIIb-IIIa and GPIb, and did not definitively colocalize with Fgn/Au-occupied receptors.

Conclusions:

  • Talin's submembrane localization in activated platelets is independent of GPIIb-IIIa and GPIb.
  • Talin does not exclusively redistribute with GPIIb-IIIa, suggesting it may stabilize connections with other platelet glycoproteins.