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Updated: Jul 13, 2026
A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery
Published on: April 10, 2014
Expression of E-selectin mRNA during ischemia/reperfusion injury
K L Billups1, M A Palladino, B T Hinton
1Department of Urologic Surgery, University of Minnesota, Minneapolis 55455, USA.
Abstract:
To better understand molecular and cellular processes involved in tissue inflammation, we have examined expression of endothelial leukocyte adhesion molecule 1 (E-selectin) mRNA in adult male rats after ischemia/reperfusion (I/R) injury and after intravenous injection of lipopolysaccharide (LPS). The polymerase chain reaction was used to generate a rat E-selectin cDNA fragment by using heart total RNA from rats exposed to LPS. This partial cDNA fragment spanned sequences from complement repeat region-5 to the second cytoplasmic tail domain. Comparison of the predicted amino acid sequence from the rat cDNA fragment to mouse and human E-selectin protein sequences showed significant conservation. The rat E-selectin cDNA fragment was used as a probe to examine the regulation of E-selectin mRNA expression by Northern blot analysis. As previously described in other animal species, E-selectin mRNA expression was induced after intravenous injection of LPS. In contrast, ischemia did not induce E-selectin mRNA expression, except in the setting of I/R injury. I/R injury triggered expression of E-selectin mRNA in the kidney. These experiments represent a first in vivo examination of E-selectin mRNA expression after I/R injury and constitute an initial step in characterizing a model system for investigating inflammation in the setting of acute ischemic injury.
Insights
Ischemia/reperfusion (I/R) injury, but not ischemia alone, induced endothelial leukocyte adhesion molecule 1 (E-selectin) mRNA expression in rat kidneys. This study provides a model for investigating inflammation during acute ischemic injury.
Area of Science:
- Molecular biology
- Immunology
- Physiology
Background:
- Tissue inflammation involves complex molecular and cellular processes.
- Endothelial leukocyte adhesion molecule 1 (E-selectin) plays a role in inflammatory responses.
- Understanding E-selectin regulation is crucial for studying inflammation.
Purpose of the Study:
- To investigate the expression of E-selectin mRNA in adult male rats following ischemia/reperfusion (I/R) injury.
- To compare E-selectin mRNA expression after I/R injury versus lipopolysaccharide (LPS) injection.
- To establish a model for studying inflammation in acute ischemic injury.
Main Methods:
- Polymerase chain reaction (PCR) was used to generate a rat E-selectin cDNA fragment.
- Northern blot analysis was employed to examine E-selectin mRNA expression.
- Adult male rats were subjected to I/R injury or LPS injection.
Main Results:
- A conserved rat E-selectin cDNA fragment was successfully generated and characterized.
- LPS injection induced E-selectin mRNA expression, consistent with previous studies.
- I/R injury, specifically in the kidney, triggered E-selectin mRNA expression, while ischemia alone did not.
Conclusions:
- E-selectin mRNA expression is induced by I/R injury in the kidney.
- This study establishes a novel in vivo model for examining inflammation in acute ischemic injury.
- The findings contribute to understanding the molecular mechanisms of inflammation in ischemic conditions.
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