Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adhesive interactions between alternatively spliced CD44 isoforms

A Droll1, S T Dougherty, R K Chiu

  • 1Terry Fox Laboratory for Hematology/Oncology, British Columbia Cancer Agency, Vancouver, Canada.

The Journal of Biological Chemistry
|May 12, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Search for New Physics in Jet Multiplicity Patterns of Multilepton Events at sqrt[s]=13  TeV.

Physical review letters·2025
Same author

Observation of Λ Hyperon Local Polarization in p-Pb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2025
Same author

Evidence for Similar Collectivity of High Transverse-Momentum Particles in p-Pb and Pb-Pb Collisions.

Physical review letters·2025
Same author

Search for a Neutral Gauge Boson with Nonuniversal Fermion Couplings in Vector Boson Fusion Processes in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2025
Same author

Reweighting simulated events using machine-learning techniques in the CMS experiment.

The European physical journal. C, Particles and fields·2025

CD44 isoforms CD44H and CD44R1 bind hyaluronan, but only CD44R1 promotes cell aggregation. CD44R1 recognizes a determinant distinct from the hyaluronan-binding site, influencing cell adhesion.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Immunology

Background:

  • Alternative splicing of the CD44 gene generates numerous isoforms with varying functions.
  • The impact of specific inserted domains on CD44 ligand-binding specificity is not well understood.

Purpose of the Study:

  • To investigate the functional differences between CD44 isoforms, specifically CD44H and CD44R1.
  • To determine the effect of CD44 isoforms on cellular aggregation and ligand binding.

Main Methods:

  • Transduction of CD44-negative murine lymphoma cells (TIL1) with different CD44 isoforms.
  • Adhesion assays using modified TIL1 cells and COS7 cells transfected with CD44R1.
  • Monoclonal antibody blocking studies to identify binding determinants.

Related Experiment Videos

Main Results:

  • Both CD44H and CD44R1 bind hyaluronan, but only CD44R1 promotes homotypic cellular aggregation in TIL1 cells.
  • CD44R1 recognizes a common determinant on both CD44H and CD44R1.
  • This determinant recognized by CD44R1 is located in a region distinct from the hyaluronan-binding site.

Conclusions:

  • CD44R1 possesses unique properties that promote cellular aggregation, independent of its hyaluronan-binding function.
  • The distinct binding determinant recognized by CD44R1 plays a crucial role in mediating cell-cell interactions.