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Updated: Jul 13, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Recovery and phylogenetic analysis of novel archaeal rRNA sequences from a deep-sea deposit feeder
J O McInerney1, M Wilkinson, J W Patching
1Department of Microbiology, University College Galway, Ireland.
Abstract:
In 1992, two independent reports based on small-subunit rRNA gene (SSU rDNA) cloning revealed the presence of novel Archaea among marine bacterioplankton. Here, we report the presence of further novel Archaea SSU rDNA sequences recovered from the midgut contents of a deep-sea marine holothurian. Phylogenetic analyses show that these abyssal Archaea are a paraphyletic component of a highly divergent clade that also includes some planktonic sequences. Our data confirm that this clade is a deep-branching lineage in the tree of life.
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