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Visualization of INT2 and HST1 amplification in oral squamous cell carcinomas
C M Lese1, K M Rossie, B N Appel
1Department of Human Genetics, University of Pittsburgh, Pennsylvania, USA.
Genes, Chromosomes & Cancer
|April 1, 1995
Summary
Gene amplification at chromosome 11q13, specifically INT2 and HST1, is linked to oral squamous cell carcinoma (OSCC) development. This genetic alteration appears to be a late event in OSCC tumorigenesis.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Oral squamous cell carcinoma (OSCC) progresses through genetic alterations, including tumor suppressor gene loss and oncogene changes.
- Band 11q13 amplification is frequently observed in head and neck squamous cell carcinomas.
Purpose of the Study:
- To investigate the amplification of INT2 and HST1 genes located at band 11q13 in OSCC cell lines and tumor tissues.
- To confirm the chromosomal localization of gene amplification in OSCC.
Main Methods:
- Classical and molecular cytogenetic analysis of seven OSCC cell lines.
- Dual-color fluorescence in situ hybridization (FISH) using DNA probes for INT2, HST1, and the chromosome 11 centromere.
- Analysis of fresh tumor and adjacent oral mucosa samples.
Main Results:
- Homogeneously staining regions (hsrs) indicating amplification were found in four of seven OSCC cell lines.
- Coamplification of INT2 and HST1 was detected in hsrs of cultured tumor cells and in fresh tumor cells from two patients.
- Amplification was absent in tumors without hsrs and in adjacent oral mucosa.
Conclusions:
- The presence of INT2/HST1 amplification in fresh tumor cells suggests it occurs in vivo and may be crucial for OSCC development or progression.
- 11q13 amplification appears to be a late-stage event in the multistep development of OSCC.