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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
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Complete removal of mycoplasma from viral preparations using solvent extraction
M La Linn1, A J Bellett, P G Parsons
1Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Abstract:
Mycoplasma contamination of two alphavirus isolates and adenovirus 5 was completely removed using a very simple, rapid protocol based on solvent extraction. Treated virus preparations remained free of mycoplasma after 6 months in culture.
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