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Related Experiment Videos

Tachykinin effects on bladder activity in conscious normal rats

O Ishizuka1, A Mattiasson, K E Andersson

  • 1Department of Urology, Lund University Hospital, Sweden.

The Journal of Urology
|July 1, 1995
PubMed
Summary

Neurokinin A (NKA) instills micturition in rats via NK2 receptors. Intra-arterial NKA causes bladder hyperactivity by stimulating both NK1 and NK2 receptors, indicating distinct receptor roles in bladder function.

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Area of Science:

  • Urology
  • Neuropharmacology
  • Physiology

Background:

  • The role of tachykinins and their receptors in regulating bladder function is not fully elucidated.
  • Neurokinin A (NKA), Substance P (SP), and Neurokinin B (NKB) are key tachykinins involved in various physiological processes.

Purpose of the Study:

  • To investigate the role of NKA, SP, and NKB in initiating micturition and bladder contractility in rats.
  • To determine the specific neurokinin (NK) receptor subtypes involved in these effects.

Main Methods:

  • Intravesical and intra-arterial administration of NKA, SP, and NKB in normal, unanesthetized rats.
  • Administration of selective NK1 and NK2 receptor antagonists (SR 48,968, MEN 10,627, RP 67,580, SR 140,333) to block receptor activity.
  • Measurement of micturition initiation and intravesical pressure to assess bladder activity.

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Main Results:

  • Intravesical NKA, but not SP or NKB, stimulated micturition, an effect blocked by NK2 receptor antagonists.
  • Intra-arterial NKA increased basal intravesical pressure, indicating direct detrusor smooth muscle contraction, an effect blocked by combined NK1 and NK2 antagonists.
  • Intra-arterial NKB weakly stimulated micturition, while intra-arterial SP had minimal effects.

Conclusions:

  • Intravesical NKA initiates micturition in rats via stimulation of urothelial NK2 receptors.
  • Intra-arterial NKA induces bladder hyperactivity through the activation of both NK1 and NK2 receptors in the bladder.
  • These findings highlight distinct receptor-mediated mechanisms for NKA in regulating bladder function depending on the route of administration.