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Gene targeting for inflammatory cell adhesion molecules

D C Bullard1, E T Sandberg, K Scharffetter-Kochanek

  • 1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.

Agents and Actions. Supplements
|January 1, 1995
PubMed
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Gene targeting in mice creates mutations in inflammatory cell adhesion molecules. These genetic modifications reveal crucial roles for molecules like CD18 and ICAM-1 in immune responses and leukocyte behavior.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Inflammatory cell adhesion molecules (ICAMs) play critical roles in immune responses.
  • Understanding the function of ICAMs is essential for developing new therapeutic strategies.
  • Gene targeting offers a powerful method to study gene function in vivo.

Purpose of the Study:

  • To investigate the functional roles of various inflammatory cell adhesion molecules.
  • To demonstrate the utility of gene targeting in creating specific mutations in ICAMs.
  • To analyze the impact of these mutations on cellular and organismal functions.

Main Methods:

  • Gene targeting in mouse embryonic stem cells to introduce specific mutations.
  • Generation of knockout mice for selected inflammatory cell adhesion molecules.

Related Experiment Videos

  • Phenotypic analysis of mutant mice to assess immune and inflammatory responses.

    • Main Results:

    • Mutations in CD18 and ICAM-1 resulted in impaired inflammatory and immune responses.
    • P-selectin and L-selectin mutations led to decreased leukocyte rolling and emigration.
    • Alpha 5 integrin mutation caused embryonic lethality, highlighting its essential role.

    Conclusions:

    • Gene targeting is a valuable tool for dissecting the functions of inflammatory cell adhesion molecules.
    • Specific ICAMs are crucial for leukocyte trafficking and immune cell function.
    • Understanding ICAM function through genetic manipulation aids in comprehending inflammatory processes.