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Forskolin enhanced off-response in the turtle olfactory system
M Kashiwayanagi1, H Kawahara, K Kurihara
1Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Journal of Physiology, Paris
|January 1, 1994
Summary
Olfactory off-responses, triggered when odor stimulation stops, are not solely dependent on the cAMP pathway. However, cyclic adenosine monophosphate (cAMP) significantly enhances these off-responses in turtles.
Area of Science:
- Neuroscience
- Olfactory System Research
- Sensory Biology
Background:
- Olfactory responses occur at both the start and end of odor stimulation.
- The cyclic adenosine monophosphate (cAMP) pathway is recognized for its role in generating odor responses.
Purpose of the Study:
- To investigate the role of the cAMP pathway in generating olfactory off-responses.
- To determine how cAMP affects the generation and modulation of off-responses in the turtle olfactory system.
Main Methods:
- Odorants were applied to turtle olfactory epithelium after saturation with forskolin.
- The effect of forskolin and 8-(4-chlorophenylthio)-adenosine 3',5'-cyclic monophosphate (cpt-cAMP) on off-responses was measured.
Main Results:
- Various odorants induced off-responses even after cAMP pathway saturation with forskolin.
- Forskolin significantly enhanced the magnitude of off-responses to certain odorants (100-1400%).
- cpt-cAMP also enhanced specific odorant-induced off-responses.
Conclusions:
- Olfactory off-responses are not exclusively mediated by the cAMP-dependent pathway.
- cAMP plays a significant modulatory role in enhancing olfactory off-responses in turtles.