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Related Experiment Videos

Differential CD44 expression patterns in primary brain tumours and brain metastases

H Li1, J Liu, M Hofmann

  • 1Division of Molecular Biology, Shenyang Medical College, People's Republic of China.

British Journal of Cancer
|July 1, 1995
PubMed
Summary

CD44 splicing variants (CD44v) promote metastasis. CD44v is rare in primary brain tumors but common in brain metastases, suggesting CD44v indicates metastatic potential in human cancers.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Splicing variants of CD44 (CD44v) are recognized as metastasis promoters in various cancers.
  • The prevalence of CD44v in human cancers and its expression in non-metastatic tumors remain unclear.

Purpose of the Study:

  • To investigate CD44 expression patterns in primary brain tumors (PBTs) and brain metastases (BMTs).
  • To determine the association between CD44v expression and metastatic potential in human malignancies.

Main Methods:

  • Utilized Northern blot, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemical analysis.
  • Examined CD44 expression in 56 PBTs and 26 BMTs from diverse origins.

Main Results:

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  • All 56 PBTs expressed standard CD44 (CD44s), but none showed CD44v expression.
  • CD44v was detected in 22 out of 26 BMTs, indicating its prevalence in metastatic brain lesions.
  • CD44v expression was found to be rare in PBTs but common in BMTs.

    • Conclusions:

    • CD44v expression is generally distributed in brain metastases but exceptionally rare in primary brain tumors.
    • The presence or absence of CD44v expression may serve as a marker for high or low metastatic potential in human cancers.