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Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using
J S Lin1, E A Burgstaler, A A Pineda
1Mayo Clinic, Division of Transfusion Medicine, Rochester, MN 55905, USA.
Abstract:
It is generally assumed that using high whole blood flow rates (WBFR), 80 ml/min, during peripheral blood stem cell (PBSC) collection on the Fenwal CS 3000 Plus blood cell processor will result in higher yields of mononuclear cells (MNC) than using lower WBFR (50 ml/min). To test this assumption, we retrospectively studied 129 PBSC procedures on 17 patients in a multiple myeloma protocol comparing MNC yield, as well as red blood cell (RBC), granulocyte, and platelet (Plt) content, of four average WBFR groups. Standard PBSC procedures were performed using modified procedure 1, interface offset 100, anticoagulant (AC) ratio of 11:1, small volume collection chamber, and a processing time of 4 hours. After correcting for AC volume used, the volume processed was divided by 240 minutes to obtain the average WBFR. WBFRs were separated into 4 groups of 40-49, 50-59, 60-69, and 70-79 ml/min. When compared to the highest flow rate group (70-79 ml/min), the three lower flow rate groups had significantly higher MNC yields of 16.2 +/- 6.9, 13.1 +/- 5.1, and 11.5 +/- 4.7 x 10(9), respectively, as compared to 8.9 +/- 6.1 x 10(9) MNC for the 70-79 ml/min group. There was no significant difference in granulocyte yield which ranged from 1.6 +/- 2.1 to 4.5 +/- 4.8 x 10(9).(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
Higher whole blood flow rates (WBFR) do not increase mononuclear cell (MNC) yields during peripheral blood stem cell (PBSC) collection. Lower WBFRs actually resulted in significantly higher MNC yields in this study.
Area of Science:
- Hematology
- Cellular Biology
- Medical Device Technology
Background:
- Peripheral Blood Stem Cell (PBSC) collection is a critical procedure.
- Optimizing collection efficiency, particularly mononuclear cell (MNC) yield, is essential.
- Assumed correlation between high whole blood flow rates (WBFR) and increased MNC yield requires investigation.
Purpose of the Study:
- To evaluate the impact of different WBFRs on MNC yield during PBSC collection.
- To compare MNC, red blood cell (RBC), granulocyte, and platelet (Plt) yields across various WBFRs.
- To challenge the assumption that higher WBFRs lead to greater MNC collection efficiency.
Main Methods:
- Retrospective analysis of 129 PBSC collection procedures on 17 patients.
- Utilized the Fenwal CS 3000 Plus blood cell processor with standardized settings.
- Grouped procedures into four average WBFR categories (40-49, 50-59, 60-69, 70-79 ml/min) after accounting for anticoagulant volume.
Main Results:
- Lower WBFR groups (40-49, 50-59, 60-69 ml/min) demonstrated significantly higher MNC yields compared to the highest WBFR group (70-79 ml/min).
- MNC yields for lower WBFR groups ranged from 11.5 to 16.2 x 10^9, versus 8.9 x 10^9 for the highest WBFR group.
- No significant differences in granulocyte yields were observed across the WBFR groups.
Conclusions:
- Contrary to assumption, lower whole blood flow rates (WBFR) are associated with higher mononuclear cell (MNC) yields in PBSC collection.
- The findings suggest that optimizing WBFR below 70 ml/min may enhance MNC collection efficiency.
- Further research could explore the mechanisms behind this inverse relationship and its clinical implications.