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Blood cell dynamics in P-selectin-deficient mice
R C Johnson1, T N Mayadas, P S Frenette
1Center for Blood Research, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Blood
|August 1, 1995
Summary
Mice lacking P-selectin show delayed neutrophil removal, leading to neutrophilia. P-selectin deficiency also impairs leukocyte rolling and macrophage recruitment in prolonged inflammation.
Area of Science:
- Immunology
- Cell Biology
Background:
- P-selectin is crucial for leukocyte adhesion to activated endothelium.
- P-selectin deficiency in mice leads to peripheral neutrophilia.
Purpose of the Study:
- Investigate the mechanisms behind P-selectin-mediated neutrophilia.
- Elucidate the role of P-selectin in leukocyte-endothelial interactions during inflammation.
Main Methods:
- Utilized P-selectin-deficient mice and wild-type controls.
- Administered epinephrine to assess neutrophil mobilization.
- Tracked clearance of 51Chromium-labeled neutrophils.
- Employed intravital microscopy to observe leukocyte-endothelial interactions.
- Induced peritonitis to evaluate macrophage recruitment.
Main Results:
- Neutrophilia in P-selectin-deficient mice is due to delayed neutrophil clearance, not altered bone marrow precursors or margination.
- Leukocyte rolling was observed in P-selectin-deficient mice but with reduced cell numbers and velocity.
- Macrophage recruitment in a peritonitis model was significantly impaired in P-selectin-deficient mice.
Conclusions:
- P-selectin plays a role beyond initial inflammatory response stages.
- P-selectin is essential for sustained leukocyte rolling and macrophage recruitment in prolonged tissue injury.