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The baculovirus p35 protein inhibits Fas- and tumor necrosis factor-induced apoptosis

D R Beidler1, M Tewari, P D Friesen

  • 1Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA.

The Journal of Biological Chemistry
|July 14, 1995
PubMed

Insights

The baculovirus p35 protein inhibits apoptosis across diverse systems, including viral, developmental, and neuronal cell death. This study demonstrates p35 also blocks Fas and tumor necrosis factor (TNF)-induced apoptosis by inhibiting prICE, a key protease.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Virology

Background:

  • The baculovirus p35 gene product is known to inhibit apoptosis induced by viruses, developmental processes, and in mammalian neuronal cells.
  • This suggests p35 targets a conserved component of the cell death machinery.

Purpose of the Study:

  • To investigate the role of p35 in Fas- and tumor necrosis factor (TNF)-induced apoptosis.
  • To determine if p35 affects the proteolytic cleavage of poly(ADP-ribose) polymerase (PARP).

Main Methods:

  • Experiments were conducted to assess the inhibitory effects of p35 on Fas- and TNF-induced apoptosis.
  • Proteolytic cleavage of PARP was analyzed in the presence and absence of p35.

Main Results:

  • p35 was shown to inhibit both Fas- and TNF-induced apoptosis.
  • p35 blocked the cleavage of PARP from its 116-kDa to 85-kDa form, a process mediated by prICE.
  • These findings suggest p35 directly or indirectly inhibits prICE.

Conclusions:

  • The results indicate that TNF- and Fas-mediated apoptotic pathways share common components with other conserved death programs.
  • p35's inhibition of prICE highlights its crucial role in regulating apoptosis across various pathways.

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