Related Experiment Videos
Stimulatory effect of ursodeoxycholate on pancreatic exocrine secretion in an in vitro study
H Shinozaki1, A Funakoshi, K Miyasaka
1Social Insurance Inatsuki Hospital, Fukuoka, Japan.
Abstract:
Ursodeoxycholate (UDCA; 300 microM) significantly stimulates amylase secretion from isolated rat pancreatic acini but not Ca2+ mobilization. Removal of extracellular Ca2+ completely abolished the UDCA-stimulated secretory response. Ca ionophore (A23187) potentiated the response. However, staurosporine, a protein kinase C inhibitor, did not affect the UDCA-stimulated amylase secretion. These results indicated that UDCA directly acted on the pancreatic acini and stimulated amylase secretion by the mechanism of stimulation of influx of extracellular Ca2+, but not through inositol [1,4,5] triphosphate and diacylglycerol formation or Ca2+ mobilization.