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Related Experiment Videos

Large secretory structures at the cell surface imaged with scanning force microscopy

A Spudich1, D Braunstein

  • 1Department of Molecular and Cellular Physiology, Stanford University, CA 94305, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 18, 1995
PubMed
Summary
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Scanning force microscopy revealed surface pits on rat basophilic leukemia (RBL-2H3) cells during secretion. These structures, approximately 1.5 microns in diameter, appear linked to the cell

Area of Science:

  • Cell Biology
  • Biophysics
  • Immunology

Background:

  • Rat basophilic leukemia (RBL-2H3) cells are a model for studying cellular secretion.
  • Surface IgE receptor cross-linking triggers secretion in RBL cells.
  • Understanding the physical mechanisms of cellular secretion is crucial.

Purpose of the Study:

  • To investigate the cell surface structures involved in RBL-2H3 cell secretion.
  • To correlate the morphology of these structures with secretion dynamics.
  • To explore the potential role of these structures in membrane retrieval post-stimulation.

Main Methods:

  • Utilized scanning force microscopy (SFM) to image RBL-2H3 cell surfaces.
  • Applied different stimulation conditions, including IgE receptor cross-linking.

Related Experiment Videos

  • Analyzed topographic changes and structure profiles over time (2, 5, 35 min post-activation).

    • Main Results:

    • Identified surface structures approximately 1.5 microns in diameter associated with secretion.
    • Observed spatial and temporal correlation between these structures and the secretion process.
    • Demonstrated persistence and changing cross-sectional profiles of these structures post-activation.

    Conclusions:

    • These surface structures are likely involved in the secretion process of RBL-2H3 cells.
    • Their characteristics suggest a potential role in membrane retrieval following degranulation.
    • SFM provides valuable insights into the dynamic physical changes during cellular secretion.