Effects of chlorpyrifos on neuronal development in rat embryo midbrain micromass cultures

Chlorpyrifos (CPF) is a broad-spectrum organophosphate insecticide used to control mosquitos and household insects. In man it has toxic effects on the central nervous system, the cardiovascular system and the respiratory system. This study investigated the toxicity of CPF on nervous system development using the midbrain micromass culture system. Chlorpyrifos was tested as a marketed formulation and in 3 solvents. All demonstrated toxicity in midbrain micromass cells with IC50 values below 30 ug/mL, indicating a potent teratogen.