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Insulin-activated amiloride-blockable nonselective cation and Na+ channels in the fetal distal lung epithelium
1MRC Group in Lung Development, Hospital for Sick Children Research Institute, University of Toronto Faculty of Medicine, Ontario, Canada.
General Pharmacology
|July 1, 1995
Abstract:
1. The apical membrane of fetal distal lung epithelium had two types of amiloride-blockable Na(+)-permeant cation channels; (1) nonselective cation (NSC) channel with a single channel conductance of 27 pS and (2) Na+ channel with a single channel conductance of 12 pS around resting membrane potential. 2. The IC50 of amiloride to the Na+ channel was 1-2 microM, while the IC50 of amiloride to the NSC channel was less 1 microM. The open probability of the Na+ channel was about 10-fold larger than that of the NSC channel. 3. Insulin (100 nM) increased the open probability of both channels.