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Cytokeratin expression in bovine corpora lutea
A M Ricken1, K Spanel-Borowski, M Saxer
1Institute of Anatomy, University of Basle, Switzerland.
Histochemistry and Cell Biology
|May 1, 1995
Summary
Cytokeratin (CK)-positive cells in bovine corpora lutea originate from the granulosa layer during the early estrous cycle. Their presence and distribution change significantly throughout the cycle and in early pregnancy.
Area of Science:
- Reproductive biology
- Cell biology
- Endocrinology
Background:
- Cytokeratin (CK)-positive cells in bovine corpora lutea exhibit endothelial-like behavior in culture.
- The cellular origin and dynamic expression of CK in the corpus luteum remain incompletely understood.
Purpose of the Study:
- To investigate the origin and distribution of Cytokeratin (CK)-positive cells within bovine corpora lutea across different estrous cycle stages and pregnancy.
- To correlate CK expression with specific luteal structures and stages.
Main Methods:
- Immunohistochemistry and Western blot analysis were performed on 45 bovine corpora lutea from various estrous cycle stages and 7 from pregnant cows.
- Tissues were staged based on morphology, weight, and progesterone content.
Main Results:
- Cytokeratin expression was detected in 26 of 45 corpora lutea, with higher prevalence in early stages.
- Early stage corpora lutea showed CK-positive cells predominantly in the granulosa layer region, while later stages exhibited varied distribution.
- CK-positive cells were associated with microvasculature in some secretory corpora lutea, resembling endothelial sprouts.
Conclusions:
- Cytokeratin-positive cells in the bovine corpus luteum originate from the granulosa layer and exhibit stage-specific expression patterns.
- The presence of CK-positive cells, particularly those associated with vasculature, may indicate a role in luteal vascularization or remodeling.