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Related Experiment Videos

Isolation of a primate embryonic stem cell line

J A Thomson1, J Kalishman, T G Golos

  • 1Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715-1299, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 15, 1995
PubMed
Summary

Researchers derived a new primate embryonic stem cell line from rhesus monkeys. This cell line, R278.5, maintains pluripotency and can differentiate into all three embryonic germ layers.

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Area of Science:

  • Stem Cell Biology
  • Primate Embryology
  • Developmental Biology

Background:

  • Embryonic stem cells (ESCs) are crucial for developmental biology research due to their pluripotency.
  • Deriving and characterizing ESCs from various species is essential for comparative studies and understanding early development.
  • Primate ESCs are particularly valuable for modeling human development and disease.

Purpose of the Study:

  • To derive and characterize a novel embryonic stem cell line from a rhesus monkey blastocyst.
  • To assess the pluripotency and differentiation potential of the derived cell line.
  • To establish the first primate ESC line for broader research applications.

Main Methods:

  • Derivation of a cloned cell line (R278.5) from a rhesus monkey blastocyst.

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  • Continuous in vitro culture and passage for over one year.
  • Karyotyping and analysis of characteristic ESC surface markers (alkaline phosphatase, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81).
  • In vitro differentiation assays with and without feeder layers and leukemia inhibitory factor.
  • In vivo differentiation studies following injection into severe combined immunodeficient (SCID) mice.

    • Main Results:

    • The R278.5 cell line maintained undifferentiated status for over a year with a normal XY karyotype.
    • Cells expressed key markers characteristic of human embryonal carcinoma cells and ESCs.
    • R278.5 cells required feeder layers for maintaining pluripotency, differentiating or dying without them.
    • In vitro differentiation induced secretion of chorionic gonadotropin and expression of alpha-fetoprotein mRNA.
    • In vivo studies demonstrated differentiation into derivatives of all three embryonic germ layers.

    Conclusions:

    • The R278.5 cell line represents a stable, pluripotent embryonic stem cell line derived from a primate.
    • This marks the first derivation of an ESC line from any primate species.
    • The R278.5 line provides a valuable tool for studying primate development, stem cell biology, and disease modeling.