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Related Experiment Videos

Aromatic-degrading Sphingomonas isolates from the deep subsurface

J K Fredrickson1, D L Balkwill, G R Drake

  • 1Pacific Northwest Laboratory, Richland, Washington 99352, USA.

Applied and Environmental Microbiology
|May 1, 1995
PubMed
Summary
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Microbial ecology·2013

Bacteria in the Sphingomonas genus were found in Southeast Coastal Plain subsurface sediments. Many of these Sphingomonas bacteria can degrade a wide range of aromatic compounds, indicating a common capability in this environment.

Area of Science:

  • Environmental microbiology
  • Bacterial taxonomy and phylogeny
  • Bioremediation

Background:

  • Subsurface sediments harbor diverse microbial communities.
  • Aromatic compounds are common environmental pollutants.
  • Understanding microbial degradation pathways is crucial for bioremediation.

Purpose of the Study:

  • To characterize a toluene- and naphthalene-degrading bacterium (strain F199) from Southeast Coastal Plain subsurface sediments.
  • To determine the phylogenetic relationship of strain F199 and related isolates.
  • To assess the aromatic compound degradation capabilities of Sphingomonas species from this environment.

Main Methods:

  • 16S rRNA nucleotide base sequence analysis for phylogenetic identification.
  • Cellular lipid composition analysis to identify characteristic biomarkers.

Related Experiment Videos

  • Growth studies on various aromatic compounds.
  • [14C]toluene and [14C]naphthalene mineralization assays.

    • Main Results:

    • Strain F199 possesses characteristic Sphingomonas fatty acids and sphingolipids.
    • Phylogenetic analysis places F199 closest to Sphingomonas capsulata.
    • Five additional isolates are closely related to F199 and share similar lipid profiles.
    • Four of the five related isolates can degrade a broad range of aromatic compounds, including toluene and naphthalene.
    • Sphingomonas capsulata and Sphingomonas paucimobilis showed no ability to degrade aromatic compounds.

    Conclusions:

    • Bacteria of the genus Sphingomonas are prevalent in Southeast Coastal Plain subsurface sediments.
    • The capacity for degrading diverse aromatic compounds is common among Sphingomonas species in this specific subsurface environment.
    • These findings highlight the potential of Sphingomonas from this region for bioremediation applications.