Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction of rat liver lysosomes with basic polypeptides

E V Rukavishnikova1, T A Korolenko, T Sassa

  • 1Department of Nutritional Chemistry, School of Medicine, University of Tokushima, Japan.

FEBS Letters
|August 7, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutron-Multiplicity Measurement in Muon Capture on Oxygen Nuclei in the Gadolinium-Loaded Super-Kamiokande Detector.

Physical review letters·2026
Same author

First Joint Oscillation Analysis of Super-Kamiokande Atmospheric and T2K Accelerator Neutrino Data.

Physical review letters·2025
Same author

Topological charge transport by mobile dielectric-ferroelectric domain walls.

Science advances·2019
Same author

Evidence for solitonic spin excitations from a charge-lattice-coupled ferroelectric order.

Science advances·2018
Same author

Ultrafast polarization control by terahertz fields via π-electron wavefunction changes in hydrogen-bonded molecular ferroelectrics.

Scientific reports·2018
Same author

Strong polarization switching with low-energy loss in hydrogen-bonded organic antiferroelectrics.

Chemical science·2018

Basic polypeptides like polylysine disrupt lysosomal membranes, causing enzyme leakage and aggregation. Adenosine triphosphate (ATP) stabilizes lysosomes against these effects, offering insights into lysosome-protein interactions.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Lysosomes are crucial cellular organelles involved in degradation and recycling.
  • Understanding lysosomal membrane integrity is vital for cellular health.
  • Protein interactions with cellular membranes can significantly alter function.

Purpose of the Study:

  • To investigate the interaction of lysosomes with various polypeptides.
  • To determine the effects of different polypeptides on lysosomal membrane and matrix.
  • To explore the potential stabilizing role of ATP in lysosomal function.

Main Methods:

  • In vitro incubation of rat liver lysosomes with different polypeptides (basic and acidic).
  • Assessment of lysosomal membrane and matrix markers' equilibrium densities.

Related Experiment Videos

  • Electron microscopy to observe lysosomal aggregation.
  • Evaluation of ATP's effect on lysosomal stability.

    • Main Results:

    • Basic polypeptides (polylysine, protamine) caused significant lysosomal membrane alteration and enzyme leakage.
    • Electron microscopy showed marked aggregation of lysosomes upon addition of basic polypeptides.
    • Acidic polypeptide (polyglutamic acid) did not induce these effects.
    • ATP demonstrated a stabilizing effect on lysosomes, especially in the presence of basic polypeptides.

    Conclusions:

    • Basic polypeptides disrupt lysosomal integrity, leading to enzyme release and aggregation.
    • Lysosomal response is dependent on the charge of the interacting polypeptide.
    • ATP can protect lysosomes from damage induced by basic polypeptides, highlighting a potential therapeutic avenue.