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Related Experiment Videos

Characterization of human CD7 transgenic mice

L E Schanberg1, D M Lee, D E Fleenor

  • 1Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1995
PubMed
Summary

Human CD7 gene expression in transgenic mice revealed hematopoietic expression and mitogenic induction. The CD7 transgene

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • CD7 is a transmembrane glycoprotein found on T lymphocytes and NK cells.
  • Human CD7 shares structural similarities with mouse Thy-1.
  • The functional homology between human CD7 and mouse Thy-1 is under investigation.

Purpose of the Study:

  • To investigate the tissue-specific expression of the human CD7 gene.
  • To utilize transgenic mice to study CD7 gene regulation by its own promoter.
  • To compare the expression pattern of human CD7 in mice with that of mouse Thy-1 and human CD7.

Main Methods:

  • Construction of transgenic mice carrying the human CD7 gene.
  • Analysis of human CD7 expression in various tissues and cell lineages of transgenic mice.

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  • Comparison of CD7 transgene expression with endogenous CD7 and mouse Thy-1 expression patterns.

    • Main Results:

    • The human CD7 transgene directed hematopoietic expression and was induced with T cell activation.
    • Transgenic CD7 expression in mice differed from human expression, appearing on mature B lymphocytes and macrophages.
    • Transgenic CD7 expression in mice resembled mouse Thy-1 in T cells and bone marrow cells but not in brain or fibroblasts.

    Conclusions:

    • The human CD7 transgene contains sufficient regulatory regions for hematopoietic expression and mitogenic induction.
    • The expression pattern of the CD7 transgene in mice more closely resembles human CD7 than mouse Thy-1.
    • This study provides insights into the regulatory mechanisms of CD7 gene expression.