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Interferon induction in swine lymphocyte antigen-defined miniature pigs
L T Jordan1, J B Derbyshire, B A Mallard
1Department of Veterinary Microbiology and Immunology, University of Guelph, Canada.
Research in Veterinary Science
|May 1, 1995
Summary
Swine lymphocyte antigen (SLA) phenotype influences antiviral interferon responses. High antibody-response pigs and females showed higher antiviral titers, while both groups experienced reduced leucocyte counts post-induction.
Area of Science:
- Immunology
- Veterinary Science
- Genetics
Background:
- Interferon induction is crucial for antiviral defense.
- Swine lymphocyte antigen (SLA) system plays a role in immune responses.
- Antibody production varies among different SLA phenotypes.
Purpose of the Study:
- To investigate the effect of SLA-defined phenotypes on interferon induction and antiviral responses in miniature pigs.
- To analyze the influence of sex and sire on interferon-induced antiviral activity.
- To assess changes in leucocyte counts following interferon induction.
Main Methods:
- Interferon was induced in two groups of miniature pigs with differing SLA phenotypes (low vs. high antibody response) using polyinosinic:polycytidylic acid complexed with poly-L-lysine and carboxymethylcellulose.
- Antiviral titers were measured before and at 24 hours after induction.
- Total leucocyte and lymphocyte counts were determined.
Main Results:
- Antiviral titers were not significantly influenced by SLA group immediately after induction but were higher in females.
- High antibody-response pigs (Group 2) exhibited higher antiviral titers both before and 24 hours after treatment.
- Both groups showed a significant reduction in leucocyte and lymphocyte counts six hours post-induction, with Group 2 having lower baseline leucocyte counts.
Conclusions:
- SLA phenotype, particularly high antibody response, influences interferon-induced antiviral activity.
- Female pigs and specific sires demonstrate enhanced antiviral responses.
- Interferon induction leads to a transient decrease in leucocyte and lymphocyte counts.