Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Controlled, targeted, intracellular expression of ribozymes: progress and problems

J J Rossi1

  • 1Center for Molecular Biology and Gene Therapy, Loma Linda University School of Medicine, CA 92350, USA.

Trends in Biotechnology
|August 1, 1995
PubMed
Summary

Researchers are advancing ribozyme technology for gene therapy. This review highlights successes and challenges in intracellular ribozyme applications, proposing novel strategies for future development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A critical assessment of the potential of short interfering RNA therapeutics.

Drug discovery today. Technologies·2014
Same author

Current progress in the development of RNAi-based therapeutics for HIV-1.

Gene therapy·2011
Same author

Foamy combinatorial anti-HIV vectors with MGMTP140K potently inhibit HIV-1 and SHIV replication and mediate selection in vivo.

Gene therapy·2009
Same author

Stem cells, ribozymes and HIV.

Gene therapy·2009
Same author

Innate immunity confounds the clinical efficacy of small interfering RNAs (siRNAs).

Gene therapy·2009
Same author

Cholesterol paves the way for topically applied viricides.

Cell host & microbe·2009

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Therapy

Background:

  • Ribozymes show promise for intracellular applications, particularly in human gene therapy.
  • Continued innovation is crucial for enhancing ribozyme efficacy.

Purpose of the Study:

  • To review recent achievements in intracellular ribozyme applications.
  • To identify current obstacles and propose solutions for ribozyme development.

Main Methods:

  • Literature review of recent ribozyme research.
  • Analysis of successes and challenges in intracellular applications.

Main Results:

  • Significant progress in ribozyme development for gene therapy.
  • Identification of key areas for improvement: delivery, expression, co-localization, targeting, and substrate turnover.

Related Experiment Videos

Conclusions:

  • Innovative strategies are needed to overcome existing limitations in ribozyme technology.
  • Future research should focus on testable ideas for enhanced ribozyme performance in intracellular settings.