Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+ permeable AMPA/kainate channels permit rapid injurious Ca2+ entry

Y M Lu1, H Z Yin, J H Weiss

  • 1Department of Neurology, University of California, Irvine 92717-4290, USA.

Neuroreport
|May 30, 1995
PubMed
Summary

Certain central neurons, identified by kainate-stimulated cobalt uptake (Co2+(+) neurons), are highly vulnerable to AMPA/kainate receptor injury. This vulnerability stems from a high rate of calcium (Ca2+) influx into these specific neurons.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The impact of frailty on the prognosis of chronic heart failure patients treated with cardiac resynchronization therapy defibrillator implantation].

Zhonghua nei ke za zhi·2025
Same author

[Analysis of prognostic risk factors for patients with locally advanced gastric cancer in the stage ypT0~2N0M0 after neoadjuvant chemotherapy].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2024
Same author

[Short-term efficacy and safety of cardiac contractility modulation in patients with heart failure].

Zhonghua xin xue guan bing za zhi·2024
Same author

[The efficacy and safety of laparoscopic radical gastrectomy after neoadjuvant chemotherapy combined with immunotherapy and targeted therapy].

Zhonghua yi xue za zhi·2024
Same author

Clinical outcomes of SMILE and WFG-LASIK used to treat myopia and astigmatism: A systematic review and meta-analysis.

Journal francais d'ophtalmologie·2024
Same author

[Analysis of factors related to systemic embolism in patients≥75 years old with non-valvular atrial fibrillation].

Zhonghua nei ke za zhi·2023

Area of Science:

  • Neuroscience
  • Cell Biology
  • Neuropharmacology

Background:

  • Subsets of central neurons express Ca2+ permeable AMPA/kainate channels.
  • These specific neurons are identified by kainate-stimulated cobalt (Co2+) uptake, termed Co2+(+) neurons.
  • Co2+(+) neurons exhibit unusual vulnerability to AMPA/kainate receptor-mediated excitotoxicity.

Purpose of the Study:

  • To investigate the underlying mechanisms of Co2+(+) neuron vulnerability to kainate.
  • To quantify Ca2+ influx rates in Co2+(+) neurons following kainate stimulation.
  • To compare intracellular Ca2+ ([Ca2+]i) changes with Ca2+ influx rates.

Main Methods:

  • Utilized kainate exposure to selectively lesion Co2+(+) neurons.
  • Measured kainate-triggered 45Ca2+ influx in neurons.

Related Experiment Videos

  • Employed fura-2 Ca2+ imaging to assess intracellular Ca2+ ([Ca2+]i) dynamics.

    • Main Results:

    • Brief kainate exposure selectively destroyed Co2+(+) neurons.
    • Kainate triggered a significantly higher rate of Ca2+ influx in Co2+(+) neurons compared to other neurons.
    • While low kainate concentrations preferentially elevated [Ca2+]i in Co2+(+) neurons, high concentrations caused comparable [Ca2+]i rises in all neurons, suggesting [Ca2+]i may not accurately reflect influx rates.

    Conclusions:

    • The heightened vulnerability of Co2+(+) neurons is attributed to a high rate of agonist-triggered Ca2+ influx.
    • Intracellular Ca2+ ([Ca2+]i) elevations may not reliably indicate the magnitude of Ca2+ influx.
    • This highlights a critical distinction between Ca2+ influx and intracellular Ca2+ dynamics in neuronal excitotoxicity.