Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypothalamic nitric oxide synthase gene expression is regulated by thyroid hormones

Y Ueta1, A Levy, H S Chowdrey

  • 1Department of Medicine, University of Bristol, Bristol Royal Infirmary, United Kingdom.

Endocrinology
|October 1, 1995
PubMed
Summary

Thyroid hormones significantly impact nitric oxide synthase (NOS) gene expression in the rat hypothalamus. Hypothyroidism reduces NOS transcripts, while hyperthyroidism increases them, demonstrating thyroid status

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease.

Leukemia & lymphoma·2000
Same author

[Severe obstetric complications nescessitating hospitalization and intensive care: a ten year retrospective study].

Annales francaises d'anesthesie et de reanimation·2000
Same author

The effects of acute and chronic stresses on vasotocin gene transcripts in the brain of the rainbow trout (Oncorhynchus mykiss).

Journal of neuroendocrinology·2000
Same author

Mitosis and apoptosis in the pituitary gland: tumour formation or hyperplasia?

Bailliere's best practice & research. Clinical endocrinology & metabolism·2000
Same author

["Stigma 2000"--is a change of direction really necessary?].

Harefuah·2000
Same author

Production of interleukin-1-like molecules by human sperm cells.

Fertility and sterility·2000

Area of Science:

  • Neuroendocrinology
  • Molecular Endocrinology
  • Thyroid Research

Background:

  • Thyroid hormones play crucial roles in regulating various physiological processes.
  • Nitric oxide synthase (NOS) is a key enzyme involved in neurotransmission and vascular function.
  • The regulation of NOS gene expression in the hypothalamus by thyroid status is not well understood.

Purpose of the Study:

  • To investigate the effects of altered thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON).

Main Methods:

  • Male rats were subjected to hypothyroidism (propylthiouracil-induced) and hyperthyroidism (T3 administration).
  • Quantitative in situ hybridization histochemistry was used to measure NOS gene transcripts in the PVN and SON.

Related Experiment Videos

  • Osmotic stimulation via chronic salt loading was employed to assess the interplay between thyroid status and NOS regulation.

    • Main Results:

    • Hypothyroidism significantly reduced NOS gene transcripts in the PVN and SON.
    • Administration of T3 prevented the reduction in NOS transcripts during hypothyroidism.
    • Hyperthyroidism markedly increased NOS gene transcripts in both nuclei.
    • PTU-induced hypothyroidism attenuated the up-regulation of NOS transcripts induced by osmotic stimulation.

    Conclusions:

    • Thyroid status exerts a major regulatory effect on NOS gene expression within the hypothalamic PVN and SON.
    • These findings highlight a significant interaction between thyroid hormones and the nitric oxide signaling pathway in the hypothalamus.