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Engineering a peptide epitope display system on filamentous bacteriophage
R N Perham1, T D Terry, A E Willis
1Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK.
FEMS Microbiology Reviews
|August 1, 1995
Summary
Engineered bacteriophage fd displays foreign peptides, mimicking HIV epitopes and eliciting neutralizing antibodies. This phage display system offers a novel, cost-effective vaccine production method.
Area of Science:
- Molecular Biology
- Immunology
- Virology
Background:
- Bacteriophage fd genome engineered for foreign peptide display.
- Peptides displayed on major coat protein N-terminus or in hybrid virions.
Purpose of the Study:
- To engineer bacteriophage fd for displaying foreign peptide sequences.
- To assess the structural integrity and immunogenicity of displayed peptides.
- To evaluate the potential of bacteriophage display for vaccine development.
Main Methods:
- Genome engineering of bacteriophage fd.
- X-ray diffraction for structural analysis.
- Proteolytic enzyme susceptibility assays.
- Immunization studies in mice with displayed peptides and T cell epitopes.
Main Results:
- Successful display of foreign peptides without significant structural disruption.
- Displayed V3 loop peptides mimic HIV-1MN gp120 epitopes.
- Elicitation of virus-neutralizing antibodies and T cell responses.
Conclusions:
- Bacteriophage display system effectively mimics epitopes and elicits immune responses.
- System demonstrates potential for studying immunological recognition.
- Offers an inexpensive and simple route for effective vaccine production.