Related Experiment Videos
Identification of a new acute phase protein
1Department of Biochemistry and Biophysics, Iowa State University, Ames 50011, USA.
The Journal of Biological Chemistry
|September 22, 1995
Summary
Mouse SIP24 protein, now identified as 24P3, is a secreted lipocalin. This study reveals SIP24/24P3 as a novel type 1 acute phase protein, significantly upregulated in liver and serum during inflammation.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- SIP24 protein is a secreted, inducible protein from quiescent mouse cells.
- SIP24 production is triggered by various factors like serum, growth factors, and hormones.
- The 24P3 cDNA, a lipocalin family member, was previously cloned from mouse kidney cells.
Purpose of the Study:
- To identify the protein product of mouse 24P3 mRNA.
- To investigate the role of SIP24/24P3 in the acute phase response.
- To characterize the expression patterns and regulation of SIP24/24P3.
Main Methods:
- mRNA and protein analysis in response to inflammatory stimuli.
- Tissue distribution studies in mice.
- Cell-based assays using BNL cells stimulated with TNF-alpha.
Main Results:
- SIP24 is confirmed as the product of mouse 24P3 mRNA.
- SIP24/24P3 levels dramatically increase in serum and liver during turpentine-induced acute phase response.
- SIP24/24P3 expression is primarily in the liver, with lower levels in brain and uterus, and is stimulated by TNF-alpha in BNL cells.
Conclusions:
- SIP24/24P3 is a novel type 1 acute phase protein.
- Its expression pattern and regulation are consistent with acute phase protein behavior.
- SIP24/24P3 plays a role in the inflammatory response.