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Alterations in leukocyte adhesion molecule expression after burn injury
F E Nwariaku1, W J Mileski, E Lightfoot
1Department of Surgery, University of Texas Southwestern Medical Center at Dallas 75235-9031, USA.
Objective:
To determine if thermal injury alters the expression of leukocyte adhesion molecules.
Design:
This is a controlled experimental animal study.
Materials And Methods:
Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4.
Measurements And Main Results:
ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03).
Conclusion:
Thermal injury alters the expression of leukocyte adhesion molecules.