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Related Experiment Videos

Estradiol and chlordecone (Kepone) decrease adenosine 3'5'-cyclic monophosphate concentrations in the ovariectomized

D C Johnson1, S Banerjee, S Chatterjee

  • 1Department of Obstetrics & Gynecology, University of Kansas Medical Center, R. L. Smith Research Center, Kansas City 66160-7338, USA.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
|October 1, 1995
PubMed
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Chronic exposure to estradiol benzoate or chlordecone significantly reduces uterine cyclic adenosine monophosphate (cAMP) in rats. This estrogen receptor-dependent effect highlights a complex relationship between estrogens and uterine cAMP levels.

Area of Science:

  • Endocrinology
  • Reproductive Biology
  • Molecular Pharmacology

Background:

  • Adenosine 3',5'-cyclic monophosphate (cAMP) is known to mimic some estrogenic actions in the rat uterus.
  • The precise relationship between estrogens and uterine cAMP levels remains a subject of scientific debate and investigation.

Purpose of the Study:

  • To investigate the effect of chronic exposure to estradiol benzoate (EB) and the xenoestrogen chlordecone on uterine cAMP content in ovariectomized immature rats.
  • To determine if the observed changes in cAMP are mediated through the estrogen receptor and to rule out enzyme degradation as a cause.

Main Methods:

  • Ovariectomized immature rats were chronically exposed (3 days) to estradiol benzoate (EB) or chlordecone.
  • Uterine cAMP content was measured at 48 and 72 hours.

Related Experiment Videos

  • The effect of forskolin on uterine adenylyl cyclase and the impact of the anti-estrogen ICI-182,780 were assessed.

    • Main Results:

    • Both EB and chlordecone, at doses causing equal uterine weight increase, induced equivalent decreases in uterine cAMP content.
    • The reduction in cAMP was evident by 48 hours and more pronounced by 72 hours.
    • Forskolin-stimulated cAMP production was unaffected, and ICI-182,780 dose-dependently blocked the cAMP-lowering effects, indicating estrogen receptor mediation.

    Conclusions:

    • Chronic exposure to potent estrogens like EB and chlordecone leads to a significant decrease in uterine cAMP levels in rats.
    • This reduction in cAMP is dependent on estrogen receptor activation.
    • The physiological implications of decreased uterine cAMP following chronic estrogenic stimulation require further elucidation.