Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Somatic hypermutation

M S Neuberger, C Milstein

    Current Opinion in Immunology
    |April 1, 1995
    PubMed
    Summary
    This summary is machine-generated.

    Immunoglobulin genes undergo hypermutation to create secondary response antibodies. Transgenic mice studies reveal cis-acting elements and intrinsic features driving this process, independent of antigen selection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hybrid hybridomas and the production of bi-specific monoclonal antibodies.

    Immunology today·2014
    Same author

    Continuous cultures of fused cells secreting antibody of predefined specificity. 1975.

    Journal of immunology (Baltimore, Md. : 1950)·2005
    Same author

    Production of antigen-specific human monoclonal antibodies: comparison of mice carrying IgH/kappa or IgH/kappa/lambda transloci.

    BioTechniques·2002
    Same author

    Novartis Medal Lecture. Antibodies: a paradigm for the evolution of molecular recognition.

    Biochemical Society transactions·2002
    Same author

    Switch junction sequences in PMS2-deficient mice reveal a microhomology-mediated mechanism of Ig class switch recombination.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Epstein-Barr virus and the somatic hypermutation of immunoglobulin genes in Burkitt's lymphoma cells.

    Journal of virology·2001
    Same journal

    A blind spot of human T cell immunology: epitope specificity in secondary lymphoid organs.

    Current opinion in immunology·2026
    Same journal

    Germinal center responses at barrier organ sites.

    Current opinion in immunology·2026
    Same journal

    Ocular sarcoidosis: from clinical signs to targeted interventions.

    Current opinion in immunology·2026
    Same journal

    On or within: spatial determinants of antigen handling in the nasal turbinates.

    Current opinion in immunology·2026
    Same journal

    Decoding the complexity of intestinal immunity with spatial transcriptomics.

    Current opinion in immunology·2026
    Same journal

    Reconsidering the immunological aspects of solid-phase assays for antiphospholipid antibodies detection.

    Current opinion in immunology·2026
    See all related articles

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Antibody affinity maturation is crucial for adaptive immunity.
    • Somatic hypermutation introduces point mutations into immunoglobulin genes.
    • Antigenic selection favors B cells with higher affinity antibodies.

    Purpose of the Study:

    • To investigate the cis-acting elements controlling immunoglobulin gene hypermutation.
    • To analyze the intrinsic features of hypermutation independent of antigenic selection.
    • To elucidate the molecular mechanisms underlying antibody diversification.

    Main Methods:

    • Utilized transgenic mouse models.
    • Analyzed immunoglobulin gene sequences.
    • Assessed mutation patterns and frequencies.

    Related Experiment Videos

    Main Results:

    • Identified specific cis-acting elements that regulate hypermutation.
    • Characterized intrinsic features of the hypermutation process.
    • Demonstrated that hypermutation can occur independently of strong antigenic selection.

    Conclusions:

    • Cis-acting elements play a key role in directing antibody gene hypermutation.
    • Understanding intrinsic hypermutation mechanisms is vital for antibody engineering.
    • This research provides new insights into B cell development and antibody responses.