Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent advances in differential display

P Liang1, A B Pardee

  • 1Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Current Opinion in Immunology
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The value of spectral CT-based radiomics in preoperative prediction of lymph node metastasis of advanced gastric cancer].

Zhonghua yi xue za zhi·2020
Same author

Mapping the Brain's electric fields with Magnetoelectric nanoparticles.

Bioelectronic medicine·2020
Same author

Discovery of an Aedes (Stegomyia) albopictus population and first records of Aedes (Stegomyia) aegypti in Canada.

Medical and veterinary entomology·2019
Same author

[A functional magnetic resonance imaging study of exercise addiction].

Zhonghua yi xue za zhi·2019
Same author

[Relationship between one-stop CT spectral perfusion imaging parameters and expression of lymphatic microvessel density and vascular endothelial growth factor-C in axillary lymph nodes of rabbit VX2 breast cancer].

Zhonghua yi xue za zhi·2019
Same author

[Application of a multi-material artifact reduction algorithm in a wide-detector CT in the evaluation of the portal venous angiography of postoperative TIPS and embolization].

Zhonghua yi xue za zhi·2019
Same journal

A blind spot of human T cell immunology: epitope specificity in secondary lymphoid organs.

Current opinion in immunology·2026
Same journal

Germinal center responses at barrier organ sites.

Current opinion in immunology·2026
Same journal

Ocular sarcoidosis: from clinical signs to targeted interventions.

Current opinion in immunology·2026
Same journal

On or within: spatial determinants of antigen handling in the nasal turbinates.

Current opinion in immunology·2026
Same journal

Decoding the complexity of intestinal immunity with spatial transcriptomics.

Current opinion in immunology·2026
Same journal

Reconsidering the immunological aspects of solid-phase assays for antiphospholipid antibodies detection.

Current opinion in immunology·2026
See all related articles

Differential display and RNA arbitrary primed polymerase chain reaction (RT-PCR) identify gene expression changes. Efforts focus on refining these methods to reduce false positives for accurate results.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Differential display and RNA arbitrary primed polymerase chain reaction (RT-PCR) are key techniques for identifying differentially expressed genes.
  • These methods have undergone modifications to improve efficiency and reliability.

Purpose of the Study:

  • To review and discuss the methodological advancements in differential display and RT-PCR.
  • To highlight strategies for minimizing false positives in gene expression analysis.

Main Methods:

  • Review of literature on differential display and RT-PCR techniques.
  • Analysis of various approaches to enhance accuracy and reduce errors.

Main Results:

  • Methodological modifications aim to streamline differential display and RT-PCR.

Related Experiment Videos

  • Strategies to eliminate false positives include optimizing RNA preparation, northern blot confirmation, primer variation, and experimental design.

    • Conclusions:

    • Continuous refinement of differential display and RT-PCR is crucial for reliable gene expression studies.
    • Addressing false positives is a primary focus for improving the utility of these techniques in molecular biology research.