Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Influenza virus vectors

A García-Sastre1, P Palese

  • 1Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA.

Biologicals : Journal of the International Association of Biological Standardization
|June 1, 1995
PubMed
Summary

Influenza virus vectors can be engineered to express foreign pathogen epitopes. These recombinant viruses show promise for developing effective vaccines by stimulating immune responses in animals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVA1-18 neutralizing antibody protects against SARS-CoV-2 in three preclinical models.

Research square·2021
Same author

Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes.

Journal of virology·2016
Same author

Model of influenza A virus infection: dynamics of viral antagonism and innate immune response.

Journal of theoretical biology·2014
Same author

Hemagglutinin stalk-based universal vaccine constructs protect against group 2 influenza A viruses.

Journal of virology·2013
Same author

A Sendai virus-derived RNA agonist of RIG-I as a virus vaccine adjuvant.

Journal of virology·2012
Same author

Extrapulmonary tissue responses in cynomolgus macaques (Macaca fascicularis) infected with highly pathogenic avian influenza A (H5N1) virus.

Archives of virology·2010

Area of Science:

  • Virology
  • Vaccinology
  • Molecular Biology

Background:

  • Reverse genetics enables manipulation of influenza virus genomes.
  • Recombinant influenza viruses can express foreign genetic sequences.

Purpose of the Study:

  • To explore the potential of influenza virus vectors for vaccine development.
  • To assess the immunogenicity of foreign epitopes expressed by influenza virus vectors.

Main Methods:

  • Construction of influenza virus vectors by inserting foreign epitopes into viral glycoproteins.
  • Expression of polyproteins and rescue of bicistronic genes into infectious viruses.
  • Generation of recombinant viruses expressing B- and T-cell epitopes from various pathogens.

Main Results:

  • Influenza virus vectors successfully expressed foreign B- and T-cell epitopes.
  • Immunization with these vectors induced systemic and local antibody responses.
  • Cytotoxic T-cell responses against the expressed epitopes were also observed.

Conclusions:

  • Influenza virus vectors are capable of eliciting significant immune responses.
  • These vectors represent promising candidates for novel vaccine strategies against diverse diseases.

Related Experiment Videos