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Polyethylene glycol-mediated bacterial colony transformation

B T Kurien1, R H Scofield

  • 1Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Oklahoma City, USA.

Biotechniques
|June 1, 1995
PubMed
Summary

This study presents a fast and effective bacterial transformation method using polyethylene glycol/calcium chloride (PEG/CaCl2). This technique achieved high transformation efficiencies and demonstrated plasmid DNA release, supporting the transformation process.

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