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Related Experiment Videos

Site-directed mutagenesis using thermostable enzymes

F Wong1, M Komaromy

  • 1Research Institute, Palo Alto Medical Foundation, CA 94301, USA.

Biotechniques
|June 1, 1995
PubMed
Summary
This summary is machine-generated.

A modified two-primer site-directed mutagenesis method improves DNA mutation efficiency in pBluescript vectors. This enhanced technique utilizes thermostable enzymes, enabling higher reaction temperatures for successful mutagenesis.

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  • Biotechnology
  • Genetic Engineering