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Related Experiment Videos

FGF-8 isoforms differ in NIH3T3 cell transforming potential

C A MacArthur1, A Lawshé, D B Shankar

  • 1Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|July 1, 1995
PubMed
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Fibroblast growth factor (FGF)-8 isoforms exhibit varying potencies in transforming NIH3T3 cells and inducing tumors. FGF-8b is a potent oncogene, while FGF-8a and FGF-8c show weaker transformation capabilities.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Fgf-8 is frequently activated in tumors from Wnt-1 transgenic mice, suggesting its role as a proto-oncogene.
  • The Fgf-8 gene encodes multiple secreted protein isoforms with different mature amino termini due to alternative splicing.

Purpose of the Study:

  • To investigate the differential transforming potency of three Fibroblast Growth Factor (FGF)-8 isoforms (FGF-8a, FGF-8b, and FGF-8c) on NIH3T3 cells.
  • To assess the in vivo tumorigenicity of NIH3T3 cells transfected with different FGF-8 isoforms.

Main Methods:

  • Stable transfection of NIH3T3 cells with cDNAs encoding FGF-8a, FGF-8b, or FGF-8c isoforms.
  • Morphological assessment of NIH3T3 cells post-transfection.
  • Evaluation of tumorigenicity in nude mice following transfection.

Related Experiment Videos

  • Quantification of Fgf-8 mRNA and FGF-8 protein expression.

    • Main Results:

    • FGF-8b isoform transfection resulted in marked NIH3T3 cell morphological transformation and rapid tumor formation in nude mice.
    • FGF-8a and FGF-8c isoforms induced moderate morphological changes and weak tumorigenicity.
    • All transfections yielded comparable Fgf-8 mRNA and FGF-8 protein levels, indicating isoform-specific functional differences.

    Conclusions:

    • Significant differences exist in the transforming potency of FGF-8 protein isoforms on NIH3T3 cells.
    • These findings suggest that distinct FGF-8 isoforms may possess different in vivo functions, potentially influencing oncogenesis.