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Promoter analysis of the bovine gene for seminalplasmin
1Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany.
Biochimica Et Biophysica Acta
|September 19, 1995
Abstract:
In this study we mapped the transcriptional initiation site of the gene for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-specific mRNA. We showed that the TATA-box in position -30 of the SAP gene is part of a functional promoter. A 280 bp region of the 5'-flanking region exerted a strong positive effect on promoter activity. In this region we identified consensus sequences for the transcriptional control elements AP1, AP2, PEA3 and GATA.