Related Experiment Video
Updated: Sep 27, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
Promoter analysis of the bovine gene for seminalplasmin
1Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany.
Abstract:
In this study we mapped the transcriptional initiation site of the gene for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-specific mRNA. We showed that the TATA-box in position -30 of the SAP gene is part of a functional promoter. A 280 bp region of the 5'-flanking region exerted a strong positive effect on promoter activity. In this region we identified consensus sequences for the transcriptional control elements AP1, AP2, PEA3 and GATA.
Related Concept Videos
The Eukaryotic Promoter Region
Reporter Genes
Commonly used reporter...

