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Related Experiment Videos

Protein A in Staphylococcus aureus isolates from pigs

S Takeuchi1, K Matuda, K Sasano

  • 1Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Japan.

The Journal of Veterinary Medical Science
|June 1, 1995
PubMed
Summary
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Protein A was detected in Staphylococcus aureus pig tonsil isolates using three methods. Enzyme-linked immunosorbent assay (ELISA) was most effective, identifying cell-bound protein A in 84% of isolates.

Area of Science:

  • Microbiology
  • Immunology
  • Veterinary Science

Background:

  • Staphylococcus aureus is a significant pathogen in both human and animal health.
  • Protein A is a major surface virulence factor of Staphylococcus aureus.
  • Understanding Protein A prevalence in animal isolates is crucial for disease control.

Purpose of the Study:

  • To determine the presence and quantity of Protein A in Staphylococcus aureus isolates from healthy pigs.
  • To compare the efficacy of three different detection methods for Protein A.

Main Methods:

  • Analysis of 147 Staphylococcus aureus isolates from pig tonsils.
  • Detection of cell-bound Protein A using slide hemagglutination, microplate hemagglutination, and enzyme-linked immunosorbent assay (ELISA).

Related Experiment Videos

  • Quantification of cell-bound Protein A using ELISA.

    • Main Results:

    • Cell-bound Protein A was detected in 48% (slide hemagglutination), 71% (microplate hemagglutination), and 84% (ELISA) of isolates.
    • Extracellular Protein A was not detected by microplate hemagglutination.
    • ELISA indicated most isolates contained 0.8–2.2 µg/mL of Protein A at McFarland No. 3 concentration.

    Conclusions:

    • ELISA is the most sensitive method for detecting cell-bound Protein A in Staphylococcus aureus pig isolates.
    • A high prevalence of Protein A-producing Staphylococcus aureus was found in healthy pigs.
    • These findings highlight the potential role of these isolates in disease transmission.