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Activity of the yeast FLP recombinase in Arabidopsis
R V Sonti1, A F Tissier, D Wong
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139-4307, USA.
Plant Molecular Biology
|September 1, 1995
Abstract:
The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.