Related Experiment Videos
Metal-characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1
M Wakayama1, Y Miura, K Oshima
1Department of Applied Chemistry, Faculty of Engineering, Oita University, Japan.
Bioscience, Biotechnology, and Biochemistry
|August 1, 1995
Abstract:
N-Acyl-D-glutamate amidohydrolase (D-AGase) from Pseudomonas sp. 5f-1 was a zinc-metalloenzyme which contained 2.06-2.61 g.atom of Zn per mole of enzyme. The zinc atom was required for the catalytic activity and stability of the enzyme. The N-terminal amino acid sequence of Pseudomonas sp. 5f-1 D-AGase showed 32% identity to that of Alcaligenes xylosoxydans subsp. xylosoxydans A-6.